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Become A Master in HPLC Technique - (Liquid Chromatography) from V J counsaltancy -PHARMACEUTICAL Services -Training division. Free online training for all. during training i will send you training material soft copy presentation about HPLC. Become A Professional in HPLC From A to Z - Your Comprehensive guideline. The first and the lonely Chemistry HPLC course. What you'll learn This course covers the following topics: Introduction to Liquid Chromatography How HPLC Works HPLC Analysis Components of HPLC Column Chemistry – Stationary Phase System Suitability Starting HPLC System Determination The Assay Using HPLC Method Development. HPLC stands for  Typical HPLC column dimensions are Pressure range in normal analytical separations is The mobile phase used in HPLC analysis is Increase in flow rate of mobile phase leads to HPLC columns are generally made of Most commonly used HPLC detector ...

Isocratic vs. Gradient Elution Modes & Isocratic and Gradient Elution Pumping Modes  Isocratic Elution A single composition of solvents is used for the duration of the separation Later eluting peaks are broader than earlier eluting peaks because of dispersion Steps must be taken to periodically flush the column at higher solvent strength to clean it of intractable materials that build up from sample injections Gradient Elution The composition of solvents is changed either continuously or stepwise In general, peaks are sharper throughout the chromatogram when compared to isocratic elution Some separations may be achieved which are not possible using isocratic elution Chromatogram run times may be shorter when compared to isocratic elution.  Isocratic and Gradient Elution Pumping Modes Isocratic Pump A single channel pump which requires the user to pre-mix the mobile phase. Composition remains constant with time. Quaternary Low Pressure Gradient Pump A 4 channel pump which c...

Basic HPLC System Components Solvent Degasser  – removes air gases from the solvents as they flow to the HPLC pump HPLC Pump  – provides solvent flow and proportioning Autosampler   – draws samples from vials and injects them into the solvent flow provided by the pump. Detector   – responds to the separated analytes emerging from the HPLC column and produces a signal output for the software Column Oven  – houses the HPLC column and keeps a stable temperature for reproducible separations

Introduction to HPLC High Performance Liquid Chromatography (HPLC) is a process of separating components in a liquid mixture. A liquid sample is injected into a stream of solvent ( mobile phase ) flowing through a column packed with a separation medium ( stationary phase ). Sample components separate from one another by a process of  differential migration  as they flow through the column. As bands emerge from the column, flow carries them to one or more detectors which deliver a voltage response as a function of time. This is called a  chromatogram . For each peak, the time at which it emerges identifies the sample constituent with respect to a standard. The peak’s area represents the quantity.

તમામને આરોગ્ય દિવસની શુભકામનાઓ  🄷🄰🄿🄿🅈 🄸🄽🅃🄴🅁🄽🄰🅃🄸🄾🄽🄰🄻  🄷🄴🄰🄻🅃🄷 🄳🄰🅈  યાદ રાખવાની મહત્વપૂર્ણ બાબતો   1. બીપી  120/80  2. પલ્સ  70 - 100  3. તાપમાન  36.8 - 37  4. શ્વાસ 12-16  5. હિમોગ્લોબિન: પુરુષ -13.50-18  સ્ત્રી - 11.50 - 16  6. કોલેસ્ટ્રોલ: 130 - 200  7 પોટેશિયમ: 3.50 - 5  8. સોડિયમ: 135 - 145  9. ટ્રાઇગ્લિસરાઇડ્સ: 220  10. શરીરમાં લોહીનું પ્રમાણ: PCV 30-40%  11. સુગર લેવલ: બાળકો (70-130) પુખ્તો: 70 - 115  12. આયર્ન: 8-15 મિલિગ્રામ  13. શ્વેત રક્તકણો WBC: 4000 - 11000  14. પ્લેટલેટ્સ: 1,50,000 - 4,00,000  15. લાલ રક્તકણો RBC: 4.50 - 6 મિલિયન.  16. કેલ્શિયમ: 8.6 -10.3 mg/dL  17. વિટામિન D3: 20 - 50 ng/ml.  18. વિટામિન B12: 200 - 900 pg/ml.  40/50/60 વર્ષનાં વરિષ્ઠ લોકો માટે ખાસ ટીપ્સ:  1- પ્રથમ ટીપ   જો તમને તરસ ન હોય અથવા જરૂર ન હોય તો પણ હંમેશા પાણી પીવો, સૌથી મોટી સ્વાસ્થ્ય સમસ્યાઓ અને તેમાંથી મોટાભાગની સમસ્યા શ...